Data Set (knb-lter-sbc.53.4)

SBC LTER: Reef: Microsatellite markers for the giant kelp, Macrocystis pyrifera

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Data Set General Information:
Identifier: knb-lter-sbc.53.4 (in the knb Catalog System)
Alternate Identifier: DOI: 10.6073/pasta/fb0cdc593e0554a105b0e0094093cfbd
Alternate Identifier: knb-lter-sbc.53

These data report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. 180 samples of blade tissue were collected at Carpinteria Reef, Santa Barbara Channel, CA (USA) in July 2006.

Sequence data are available from GenBank. These data were published as a Technical Note in the journal Conservation Genetics in 2009.

These data were published as a technical note:

Alberto, F., A. C. Whitmer, N. C. Coelho, M. Zippay, E. Varela-Alvarez, P. T. Raimondi, D. C. Reed and E. A. Serrão. 2009. Microsatellite markers for the giant kelp Macroystis pyrifera. Conservation Genetics. 10:1915-1917 doi: 10.1007/s10592-009-9853-9

Abstract: We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean= 16.5, and gene diversity ranging from 0.469 to 0.930 (mean= 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.

Short Name: Microsatellite markers
Publication Date: 2012-04-20
Language: english
Time Period:
Detailed Data Description and Download:
Kelp microsatellite markers, 2009
characterization of microsatellite loci of the giant kelp, Macrocystis pyrifera
People and Organizations:
View complete information for all parties
Contact: Information Manager, Santa Barbara Coastal LTER [  email ]
Organization Santa Barbara Coastal LTER
Owner: Alberto, Filipe 

Data Set Citation

How to cite this data set: Alberto, F. . 2012. SBC LTER: Reef: Microsatellite markers for the giant kelp, Macrocystis pyrifera. Santa Barbara Coastal LTER. doi:10.6073/pasta/fb0cdc593e0554a105b0e0094093cfbd

Key Words and Terms

By Thesaurus:
genbank, genetic diversity, LTER, macroalgae, microsatellite loci, microsatellite locus, microsatellite marker, microsatellites, Santa Barbara Coastal
Santa Barbara Coastal LTER Habitats Kelp Forest, Reef
Santa Barbara Coastal LTER Measurement Types Community Structure, Genomics
Santa Barbara Coastal LTER Places Carpinteria Reef
LTER Controlled Vocabulary v1 genetics, long term ecological research
LTER Controlled Vocabulary v1: Core Research Areas Population Studies
NBII Biocomplexity Alleles, Kelps, Reefs
giant kelp, Macrocystis pyrifera

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Additional Metadata

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